New equipment for bidimensional electrophoresis (2DE)
New equipment for bidimensional electrophoresis (2DE)
In bidimensional electrophoresis, proteins are separated according to two independent properties in two discrete steps: (1) Isoelectric focusing (IEF), which separates proteins according to their isoelectric points (pI), and (2) SDSpolyacrylamide gel electrophoresis (SDS-PAGE), which separates them according to their molecular weights (MW). At this stage, the gels are scanned by a laser scanner with high sensitivity and accuracy. Ettan Spot Picker is a robotic system designed to accurately collect protein spots from 2-D gels for further analyses, such as digestion and protein identification by MALDI mass spectrometry (see details in annex).
The power of 2-DE as a biochemical separation technique has been recognized since its introduction in 1975. Its application, however, has become increasingly significant as a result of a number of developments in separation techniques, image analysis, and protein characterization. Applications include: proteome analysis, cell differentiation
studies, detection of disease markers in research, cancer research, etc.
Equipments aqcuired:
- Ettan IPGphor 3 for first dimension (isoelectric focusing) is a fully integrated isoelectric focusing (IEF) system that allows proteins to be separated according to their
isoelectric points. - Ettan DALTsix electrophoresis Unit for second dimension (vertical electrophoresis)
- Laser Scanner Typhoon FLA 9500, for gel scanning, will be used to scan the 2DE gels
- Ettan Spot Picker: automatic excision of the protein spots
- Amersham Imager 600 is a sensitive and robust chemiluminescence imager for high-resolution digital imaging of protein in gels and membranes (western blotting). Images can be afterward analyzed with the IMageQuant TL software to perform protein quantification, ensuring accurate and consistent results.
